Journal: Cell Reports Medicine

Article Title: A bispecific T cell engager recruits both type 1 NKT and Vγ9Vδ2-T cells for the treatment of CD1d-expressing hematological malignancies

doi: 10.1016/j.xcrm.2023.100961

Figure Lengend Snippet: CD1d-Vδ2 bsTCE-induced lysis of CD1d+ target cells is accompanied by expansion of type 1 NKT and Vγ9Vδ2-T cells in healthy donor PBMCs (A) Fold growth of MOLM-13 (n = 5) and MM.1s.CD1d (n = 7) cells after 4–7 days’ co-culture with PBMCs (PBMC:tumor ratio 10:1, mean type 1 NKT:tumor ratio 1:856 [range 10–3,046], and mean Vγ9Vδ2-T:tumor ratio 1:17 [range 5–50]) ± 50 nM CD1d-Vδ2 bsTCE. (B) Fold expansion of type 1 NKT and Vγ9Vδ2-T cells after 4–7 days’ co-culture of PBMC and MM.1s.CD1d cells (ratio 10:1) ± 50 nM CD1d-Vδ2 bsTCE (n = 7). (C and D) Specific lysis of B cells, monocytes, and MM.1s.CD1d cells (C) and correlation of PBMC Vγ9Vδ2-T cell frequency with specific lysis of MM.1s.CD1d (D) after a 16-h co-culture of PBMCs and MM.1s.CD1d cells (ratio 10:1) ± 50 nM bsTCE (n = 6). Negative control indicates medium control. Box-and-whisker plots indicate median, 25th–75th percentiles, and minimum-maximum. Two-way ANOVA with Šídák multiple comparisons test (A and B), one-way ANOVA with Tukey multiple comparison test (C), linear regression with 95% confidence bands (dotted lines) (D). See also Figure S2 .

Article Snippet: healthy human donor PBMC , Sanquin blood supply, the Netherlands , N/A.

Techniques: Lysis, Co-Culture Assay, Negative Control, Control, Whisker Assay, Comparison

Journal: Cell Reports Medicine

Article Title: A bispecific T cell engager recruits both type 1 NKT and Vγ9Vδ2-T cells for the treatment of CD1d-expressing hematological malignancies

doi: 10.1016/j.xcrm.2023.100961

Figure Lengend Snippet:

Article Snippet: healthy human donor PBMC , Sanquin blood supply, the Netherlands , N/A.

Techniques: Blocking Assay, Recombinant, Variant Assay, Control, Binding Assay, Red Blood Cell Lysis, Isolation, Cell Isolation, Expressing, Software, FCAP Assay